Tuesday, March 29, 2005
Things don't like me today.
Went to lab, found out i digested my plasmid with the wrong r.e's
OK, fine.
Then I started on my gel shift. In a state of confusion, i mixed up my samples.
Haiya it happens lar
Set up my gel in the cold room, stupid gel started to leak. Result: one very wet floor, and loads of agarose to seal it.
its just not my day. delayed my exit time from the lab by 30 mins. okay lar 30 mins.
Because of freaky (warm) temp, (unadjusted) lab heater went crazy and I was melting in my lab coat
buay tahan , back to NS liao izzit.
Started on my protein gel. Weird sediment on my samples, I heck care and just loaded all the darn thing. Then I asked Boss about it. Result: Very possible screw up.
Me and protein gels, always no karma wan nar *shrug*
Gel shift finish liao, marker never move properly. Will know whether i screwed up tomorrow.
Wah this time siao liao. I wonder what will be the boss's face when he finds out.
Develop gelshift in darkroom. Wanted to take off my gloves, because they were stinking. Tried to take out with great difficulty, because my hands were sweaty. *Bang!* Gloves disintegrated, I hit my hand on the darkroom dorr. Now it looks like Dracula has left its mark on my hand.
Wah even the darkroom door also buey song me ar!
Then lagi best, tomolo radiation safety people coming to check, I check my area, found out one sibeh hot spot on my bench. Bloody hell, i reached home 1.5 hours late lor! how to mug lie dat.
ARRGGGGHHH
Went to lab, found out i digested my plasmid with the wrong r.e's
OK, fine.
Then I started on my gel shift. In a state of confusion, i mixed up my samples.
Haiya it happens lar
Set up my gel in the cold room, stupid gel started to leak. Result: one very wet floor, and loads of agarose to seal it.
its just not my day. delayed my exit time from the lab by 30 mins. okay lar 30 mins.
Because of freaky (warm) temp, (unadjusted) lab heater went crazy and I was melting in my lab coat
buay tahan , back to NS liao izzit.
Started on my protein gel. Weird sediment on my samples, I heck care and just loaded all the darn thing. Then I asked Boss about it. Result: Very possible screw up.
Me and protein gels, always no karma wan nar *shrug*
Gel shift finish liao, marker never move properly. Will know whether i screwed up tomorrow.
Wah this time siao liao. I wonder what will be the boss's face when he finds out.
Develop gelshift in darkroom. Wanted to take off my gloves, because they were stinking. Tried to take out with great difficulty, because my hands were sweaty. *Bang!* Gloves disintegrated, I hit my hand on the darkroom dorr. Now it looks like Dracula has left its mark on my hand.
Wah even the darkroom door also buey song me ar!
Then lagi best, tomolo radiation safety people coming to check, I check my area, found out one sibeh hot spot on my bench. Bloody hell, i reached home 1.5 hours late lor! how to mug lie dat.
ARRGGGGHHH
